Working NAS folder suddenly not accessible - Duplicati thinks it does not exist

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Exactly! Just goes to show that I have not been following the forum of late.
Indeed, the machine with the issue is on Win 10 2004 while the one that is working is on 1903
And as described, “Test connection” says “Folder … does not exist. Create it now?

However… I thought both systems were setup identically but evidently the system that is not working is running Duplicati as a service under the ‘Local system account’ while the system that is working is running the service under the main user account.I know back then, I had issues getting both system up and running as a service so it’s possible this deviation was what worked, or it was changed with the update to Win 10 2004.

I likely cannot really access that system until this evening at which point I will look more carefully at the service settings and likley add these comments to: SMB file shares not connecting - Duplicati as Service

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